Fluorescent Microscopy is established in many scientific areas as a standard method.

Nowadays, most systems use changeable optical filters and monochrome cameras for image acquisition. Since the spectral signature of fluorescent radiation is a key indicator for analytics, our hyperspectral camera systems include this dimension.

Schematic of the functionality of a fluorescent microscope; Source: [https://de.wikipedia.org/wiki/Fluoreszenzmikroskopie#/media/File:Fluoreszenzmikroskopie_2008-09-28.svg]

Schematic of the functionality of a fluorescent microscope; Source: [https://de.wikipedia.org/wiki/Fluoreszenzmikroskopie#/media/File:Fluoreszenzmikroskopie_2008-09-28.svg]

When used with one of our hyperspectral cameras, the emission filter is a changeable color filter and the detector is the camera.
For multispectral analyses, as depicted in the image below, several monochrome recordings have to be layered.

fluorescent cells; source: [https://de.wikipedia.org/wiki/Fluoreszenzmikroskopie#/media/File:FluorescentCells.jpg]

fluorescent cells; source: [https://de.wikipedia.org/wiki/Fluoreszenzmikroskopie#/media/File:FluorescentCells.jpg]

With the HSI-cameras, the spectral analysis based on the single recording is possible. Especially with live samples, which can change during the course of an analysis, this is an important and very useful feature.
Currently, our systems are used in several research projects regarding this subject. For these, our TIVITA™ Tissue is attached to a fluorescent microscope via C-Mount adapter, as shown in the image below.

Working station with fluorescent microscope and connected TIVITA™ Tissue

Working station with fluorescent microscope and connected TIVITA™ Tissue

First results show that it is possible to apply the HSI-technology in the area of fluorescence. Our research partner GMBU Jena recorded and analyzed the images below. In order to acquire the different pseudo-color images, markers have to be set in the regular color photography taken through the microscope. Via the contained spectra, different pseudo-color images can be created by using different mathematical models.

Fluorescent strip in a twenty-euro bill: Stimulation at 365 nm

Fluorescent strip in a twenty-euro bill: Stimulation at 365 nm

Cyanobacteria and protoberberine: stimulation at 470 nm

Cyanobacteria and protoberberine: stimulation at 470 nm

Weeping willow: stimulation at 470 nm

Weeping willow: stimulation at 470 nm