Die Histology (gr. histos “tissue” and logos “teachings“), is the science of biologic tissue. In a broader sense, it means microscopic anatomy. Thus, histology is a part of medicine or biology and in turn part of anatomy and pathology.

Generally, tissue samples are only assessed in the visual spectral range and by using different coloring techniques on order to create visual markers.

With our HSI cameras, you can acquire reliably and quickly a high amount and a broad spectrum of chemical information of tissue samples, because every single pixel of an image recorded with one of our HSI cameras contains the respective spectrum of the tissue sample.

Additionally, different samples can be recorded together in one hyperspectral image, thus chemometric differences can be visualized more easily.

For samples with a high water content, the optimum spectral range for analysis is VNIR. Samples with a lower water content are generally best analyzed with the NIR spectral range up to 1,7µm. The reason for this is that several spectral overtone and combination bands of the molecules can be recorded within that spectral range.

The following image shows a typical measurement setup:

HSI camera, mounted on top of a scientific microscope

HSI camera, mounted on top of a scientific microscope

 

The detailed hyperspectral image of a butterfly wing, taken with the measurement setup above, is depicted in the following image:

Schmetterlingsflügel

Image of a butterfly wing, taken with the Diaspective Vision HSI camera mounted on a scientific microscope

 

A typical workspace for hyperspectral analyses:

Diaspective Vision HSI camera during microscopic analyses

Diaspective Vision HSI camera during microscopic analyses