„Hyperspectral monitoring during microvascular surgery“

In cooperation with the Klinik und Poliklinik für Mund-, Kiefer und Plastische Gesichtschirurgie of the Rostock University, the TIVITA™ Tissue system is applied for Forun-project „Hyperspectral Monitoring during Microvascular surgery“ for measuring the perfusion of hind legs of rats.
The tisse on the inside of the rats’ thighs is opened up and the blood vessels are laid open. In a further step, the femoral artery is cut before the artery is microsurgically anastomized. The clinicians are testing new drugs, which are supposed to prevent thrombosis. At the same time, the rats’ hind legs are monitored with the TIVITA™ Tissue perioperatively, enabling a non-invasive and spatially resolved monitoring without having to use any contrast agents. Tissue oxygenation levels can be determined and depicted at different time intervals.

The following images of two laboratory animals show the progress of oxygenation in the left hind leg over time.

Progress of tissue oxygenation left hind leg Rat I

Progress of tissue oxygenation left hind leg Rat I

Progress of tissue oxygenation left hind leg Rat II

Progress of tissue oxygenation left hind leg Rat II

The images show significant differences in oxygenation. Prior to surgery, the perfusion of the tissue with oxygenated hemoglobin is high (red). This level drops completely because of the cut blood vessel and the suspended blood stream in the distal direction (blue/green). Directly after the anastomosis of the femoral artery, perfusion levels start to rise immediately and improve visibly postoperatively. Thus, the TIVITA™ Tissue is able to prove a successful anastomosis.
The animal model for the project “Hyperspectral Imaging during Microvascular Surgery” proves, that the TIVITA™ Tissue is able to diagnose hind leg ischemias, which is an established model to verify therapies for the formation of new blood vessels.

For further information, please visit our: »notes section.